Details

Source

Klebsiella pneumoniae OK 8

Reagents Supplied

CMX Buffer 1

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

No

Assay Unit Substrate

Adenovirus DNA

Reaction Buffer

CMX Buffer 1:10 mM Tris-HCl (pH 7.0 at 37°C)10 mM MgCl₂1 mM dithiothreitol

Activity in Buffers

Buffer 1:100%Buffer 2: 50%Buffer 3: <25%Buffer 4: 100%Buffer 5: 100%*Star activity

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)0.1 mM EDTA200 mM KCl1 mM DTT0.05% (v/v) TWEEN-20™500 μg/ml bovine serum albumin50% (w/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.0 at 37°C) 10 mM MgCl₂1 mM dithiothreitol1 µg Adenovirus DNA

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot