Details

Description

• The simplest DNA Polymerase known in both size and catalysis
• Replication enzyme involved in initiation of DNA replication in eukaryotes
• A multi-subunit complex, which inhibits DNA polymerase and primase activities
• Isolated from HeLa cells by immunoaffinity chromatography (1)

Applications

• Used to test in vitro replication capabilities along with other enzymes
• Used to test the toxicity of drugs on key human replication systems
• Used in DNA replication experiments

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C

Storage Buffer

20 mM Tris-HCl (pH 8.0)0.25 mM EDTA50 mM NaCl1 mM β-mercaptoethanol0.1% Triton X-100™50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Storage Conditions

Store at -80°C long termStore at -20°C short termShipped on dry ice

Downloads

Certificates of Analysis

Lot 2404003 PDF

MSDSMSDS PDF

References

(1) Podust, V.N., Lavrik, O.I., Nasheuer, H.P., and Grosse, F. (1989) FEBS Letters 245, 14-16