Details

Description

• Mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions and testing replication of mitochondrial DNA

Unit Definition

One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C

Storage Buffer

20 mM Tris-HCl (pH 8.0) 50 mM NaCl50µg/ml PMSF0.05% Triton X-100™10% DMSO5% glycerol

Assay Conditions

25 mM Hepes-KOH (pH 8.0)0.5 mM MnCl₂10 µg bovine serum albumin2.5 mMβ-mercaptoethanol100 mM NaCl10 µM TTP and 0.3µCi [3H]TTP0.29 mM poly rA:(dT)50Incubation for 15 minutes at 37°Cin a total reaction volume of 15 μl

Quality Control

All preparations are assayed for contaminating endonuclease and exonuclease activities

Storage Conditions

Store at -80°C Long Term, -20°C Short TermAvoid multiple freeze / thaw cyclesShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF