Details

Source

Escherichia coli RY 13

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C) 10 mM MgCl₂100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%Buffer 2: 100%Buffer 3: 100%Buffer 4: 75%Buffer 5: 75%*Star activity

Storage Buffer

50 mM Tris-HCl (pH 7.2 at 4°C) 300 mM NaCl0.5 mM EDTA5 mM EGTA0.2% (v/v) Triton X-100™5 mM BME5µg/ml PLL500 µg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of AnalysisLot 2404008 PDFLot 2307006 PDF

MSDSMSDS PDF