Details

Source

Desulfovibrio desulfuricans

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1μg of lambda DNA in 1 hour in a total reaction volume of 50μl

Star Activity

No

Heat Inactivation

65°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂100 mM NaCl1 mM dithiothreitol100µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%Buffer 2: 75%Buffer 3: 100%Buffer 4: 50%Buffer 5: 50%

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 4°C)100 mM NaCl0.1 mM EDTA1 mM DTT0.2% Triton X-100™50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of AnalysisLot 2207005 PDF

MSDSMSDS PDF