Details

Source

Acinetobacter calcoaceticus

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in atotal reaction volume of 50 μl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at80°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100 μg/mL bovine serum albumin

Activity in Buffers

Buffer 1: <25%Buffer 2: >75%Buffer 3: 25%Buffer 4: 100%Buffer 5: 100%

Assay Conditions

See Certificate of Analysis Below

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)10 mM BME0.1 mM EDTA0.5 mg/ml bovine serum albumin0.1% Triton X-100™50% (v/v) glycerol

Storage Conditions

Store at -20°CShipped on Dry Ice

Downloads

Certificates of AnalysisLot 2404005 PDFLot 2309003 PDF

MSDSMSDS PDF