Details

Sma I Source

Serratia marcescens

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of Adenovirus 2 DNA in 1 hour at 30°C in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

30°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-Acetate (pH 7.5 at 37°C)50 mM potassium acetate10 mM magnesium acetate100 µg/ml bovine serum albumin1 mM dithiothreitol

Activity in Buffers

Buffer 1: 0%Buffer 2:0%Buffer 3: 0%Buffer 4: 100%Buffer 5: 75%*Star activity

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 4°C)1 mM EDTA7 mM β-mercaptoethanol500 μg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

20 mM Tris‑acetate (pH 7.5 at 37°C)50 mM potassium acetate10 mM magnesium acetate100 µg/ml bovine serum albumin1 μg Adenovirus 2 DNA1 mM dithiothreitol

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot

Categories

Restriction Endonucleases