Details

Source

Xanthomonas holcicola

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂1 mM dithiothreitol50 mM NaCl100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%Buffer 2:100%Buffer 3: 100%Buffer 4: 75%Buffer 5:50%*Star activity

Storage Buffer

50 mM Tris‑HCl (pH 7.4 at 4°C) 200 mM KCl0.1 mM EDTA5 mM 2-Mercaptoethanol0.1% Triton X-100™500 µg/ml BSA50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37°C) 50 mM NaCl10 mM MgCl₂1 µg of Adenovirus 2 DNA1 mM dithiothreitol100µg/ml bovine serum albuminIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF - Current LotMSDS PDF- Current Lot

Categories

Restriction Endonucleases