Details

Source

Haemophilus influenzae Rc

Reagents Supplied

CMX Buffer 5

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 5:20 mM Tris-HCl (pH 7.0 at 37°C)5 mM MgCl₂50 mM KCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 50%Buffer 2: 50%Buffer 3: 75%Buffer 4: >75Buffer 5: 100%

Storage Buffer

10 mM Tris‑HCl (pH 7.4 at 4°C) 200 mM NaCl0.1 mM EDTA1 mM dithiothreitol500 μg/ml bovine serum albumin5 μg/ml poly-L-lysine0.1% Triton X-100™50% (v/v) glycerol

Assay Conditions

1 mM DTT100 μg/ml BSA20 mM Tris‑HCl (pH 7.0 at 37°C) 50 mM KCl5 mM MgCl₂1 μg lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 μl

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot