Details

Source

Arthrobacter luteus

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1µg of lambda DNA in 1 hour in a total reaction volume of 50µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%Buffer 2: 75%Buffer 3: 50%Buffer 4:100%Buffer 5: 50%

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)50 mM KCl0.1 mM EDTA1 mM BME500µg/ml bovine serum albumin0.1% Triton X-100™50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificates of AnalysisLot 2410002 PDF

MSDSMSDS PDF