Details
Source
Caryophanon latum L
Reagents Supplied
CMX Buffer 4
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Assay Unit Substrate
Lambda DNA
Heat Inactivation
65°C for 10 minutes
Note
Cla I is blocked by dam methylation
Reaction Buffer
CMX Buffer 4:20 mM Tris-acetate (pH 7.5 at 37°C)50 mM potassium acetate10 mM magnesium acetate1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 75%Buffer 2: 100%Buffer 3: 100%Buffer 4:100%Buffer 5: 100%
Storage Buffer
50 mM Tris-HCl (pH 7.4 at 4°C)0.1 mM EDTA200 mM KCl5 mMβ-mercaptoethanol0.1% (v/v) Triton X-100™500 µg/ml bovine serum albumin 50% (v/v) glycerol
Assay Conditions
See Certificate of Analysis Below
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of AnalysisLot 2102024 PDF
MSDSMSDS PDF
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