Details

Description

Simian virus (SV40) DNA Replication Assay Kit is the best model for mammalian chromosomal DNA replication in vitro (1) Addition of SV40 Large T Antigen initiates replication in the presence of host proteins (2)

Applications

• examining the fidelity and biochemistry of DNA replication in humans (3,4)
• identifying mode of action and mechanisms leading to cell transformation in vitro and tumor formation in vivo (5)
• regulating gene expression in eukaryotic cells (2)
• studying the inhibition of DNA replication by mutagenesis (6,7)
• determining the risks posed by human carcinogens
• comparing replication of normal and malignant cells
• investigating the cytotoxic effects of drugs (8)
• investigating apoptosis

Kit contains: HeLa cell extract, plasmids pUC-HSO and pUC-8-4, reaction buffers, creatine phosphokinase, 20x dNTPs/NTPs, phosphocreatine, yeast RNA coprecipitant Simply add SV40 Large T Antigen and you have a complete system for in vitro modeling of chromosomal DNA replication

Note

SV40 Large T Antigen sold separately

Quality Control

All preparations are assayed for stimulation of DNA synthesis in a T antigen-dependent DNA replication assay (2) Typical preparations are greater than 90% pure as judged by SDS polyacrylamide gel electrophoresis

Storage Conditions

Store at -80°CShipped on Dry Ice

Downloads

MSDS PDF

References

1. Evan, G. and Littlewood, T. (1998) Science 281,1317-13212. Wold, M.S., Weinberg, D.H., Virshup, D.M., Li, J.J. and Kelly, T.J. (1989 ) J. Biol. Chem. 264, 2801-28093. Thomas, D.C., Veaute, X., Kunkel, T.A. and Fuchs, R.P.P. (1994) Proc. Natl. Acad. Sci. 91, 7752-77564. Boyer, J.C., Thomas, D.C., Maher, V.M., McCormick, J.J. and Kunkel, T.A. (1993) Cancer Res. 53, 3270-32755. Bebenek, K., Thomas, D.C., Roberts, J.D., Eckstein, F. and Kunkel, T.A. (1993) Mol. Pharm. 43, 57-636. Stillman, B. (1994) Cell 78, 725-7287. Fanning, E. (1992) J. Virol. 66, 1289-12938. Dixon, R.A.F., and Nathans, D. (1985) J. Virol. 53, 1001-1004