Details
Sfi I Source
Streptomyces fimbriatus
Reagents Supplied
CMX Buffer 2
Incubation Temperature
50°C
Heat Inactivation
No
Assay Unit Substrate
Adenovirus 2 DNA
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Reaction Buffer
CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 25%
Buffer 2: 100%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 50%
*Star activity
Storage Buffer
5 mM potassium phosphate (pH 7.4 at 4°C)
300 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
0.15% Triton X-100™
100 µg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
10 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
50 mM NaCl
1 µg of Adenovirus 2 DNA
100 µg/ml bovine serum albumin
Incubation is at 50°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases
