Details
Rsa I Source
Rhodopseudomonas sphaeroides
Reagents Supplied
CMX Buffer 1
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 1:
10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 100%
Buffer 2: 75%
Buffer 3: 25%
Buffer 4: 75%
Buffer 5: 50%
Storage Buffer
10 mM Tris-HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
1 mg/ml bovine serum albumin
1 mM dithiothreitol
0.1 M PMSF
50% (v/v) glycerol
Assay Conditions
10 mM Tris‑HCl (pH 7.0 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases
