Details
Pvu II Source
Proteus vulgaris
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
No
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 75%
Buffer 5: 75%
*Star activity
Storage Buffer
50 mM Tris-HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
10 mM BME
500 µg/ml bovine serum albumin
0.1% Tween 20™
50% (v/v) glycerol
Assay Conditions
10 mM Tris-HCl (pH 7.5 at 37°C)
50 mM NaCl
10 mM MgCl2
1 mM dithiothrietol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Note
To avoid star activity it is not recommended to use more than 15 units per reaction or to incubate over 1 hour
Storage Conditions
Store at -20°C
Shipped on dry ice
Pvu II Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases
