Details
PvuIISource
Proteusvulgaris
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1 µgoflamBDaDNAin1hourinatotalreactionvolumeof50 µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
No
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 75%
Buffer2: 100%
Buffer3:75%
Buffer4:75%
Buffer5:75%
*Staractivity
StorageBuffer
50mMTris-HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
10mMBME
500µg/mlbovineserumalbumin
0.1%Tween20™
50%(v/v)glycerol
AssayConditions
10mMTris-HCl(pH7.5at37°C)
50mMNaCl
10mMMgCl2
1mMdithiothrietol
100µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50 µl
Note
Toavoidstaractivityitisnotrecommendedtousemorethan15unitsperreactionortoincubateover1hour
StorageConditions
Storeat-20°C
Shippedondryice
PvuIIDownloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases
