Not I Source

Nocardia otitidis-caviarum

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
100 µg/ml bovine serum albumin
1 mM dithiothreitol

Activity in Buffers

Buffer 1: 0%
Buffer 2: 50%
Buffer 3: 100%
Buffer 4: 25%
Buffer 5: 25%
*Star activity 

Storage Buffer

20 mM Tris‑HCl (pH 7.5 at 4°C)
100 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
0.01% (v/v) Triton X‑100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C) 
100 mM NaCl
10 mM MgCl₂
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1.0 µg of Adenovirus 2 DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Not I Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases