Details
NheISource
Neisseriamucosaheidelbergensis
ReagentsSupplied
CMXBuffer5
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1 µgofAdenovirus2DNAin1hourinatotalreactionvolumeof50 µl
StarActivity
Yes
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
Adenovirus2DNA
ReactionBuffer
CMXBuffer5:
20mMTris-HCl(pH7.0at37°C)
5mMMgCl2
50mMKCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 100%
Buffer2: 100%
Buffer3:75%
Buffer4:75%
Buffer5:100%
*Staractivity
StorageBuffer
10mMTris-HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
0.1mMdithiothreitol
0.1%(v/v)TritonX-100™
500µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
20mMTris-HCl(pH7.0at37°C)
50mMKCl
5mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
1µgAdenovirus2DNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
QualityControl
Allpreparationsareassayedforcontaminatingendonuclease,3"-exonuclease,andnonspecificsingle-anddouble-strandedDNaseactivities
Notes:
- Duetostaractivity,itisnotrecommendedtoincubateover4hoursortousemorethan100unitsperreaction.
- Saltconcentrationabove50mMinhibitsNheIactivity.
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases
