Details
Source
Haemophilus parainfluenzae
Reagents Supplied
CMX Buffer 4
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
No
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 25%
Buffer 2: 50%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 100%
*Star activity
Storage Buffer
20 mM Tris-HCl (pH 7.4 at 4°C)
0.5 mM EDTA
50 mM KCl
1 mM DTT
0.1% (w/v) Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
20 mM Tris‑Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
